Stigmasterol and β-Sitosterol: Antimicrobial Compounds in the Leaves of Icacina trichantha identified by GC–MS

Abstract Background The development of resistance to many antibiotics currently in use has necessitated the search for more effective alternatives. Plants have been used ethnomedicine different parts world treat various diseases. Many studies on plants confirmed their medicinal potentials and led isolation characterization several potential drug candidates. This study investigates leaves Icacina trichantha antimicrobial properties seeks identify compounds responsible observed activities. are treatment skin infections. In this study, a solvent-based fractionation bioactive was carried out using hexane, ethyl acetate, methanol. resultant extracts were screened activity against six bacteria four fungi standard procedures. GC–MS analysis most active fraction out. Results non-polar (hexane) showed better than acetate methanol extracts. At 200 mg/mL, inhibition 28.0 ± 0.82, 23.7 0.47, 24.6 0.94 Staphylococcus aureus, Escherichia coli , Bacillus subtilis, respectively. same concentration, extract zones 18.0 0.82 Candida albicans Penicillium notatum . lowest Minimum Inhibitory Concentrations (MIC) values 2.5 mg/mL obtained aureus subtilis hexane revealed 62 peaks, which ten peaks successfully characterized. Conclusions None antibacterial antifungal activities is as potent drugs, Gentamicin Tioconazole. extract, however, some microorganisms analyzed GC–MS. contained compounds, could not be identified. Two identified Stigmasterol β-Sitosterol, known possess properties. However, unidentified also contributed significantly extract.